Grand Journal of Urology
E-ISSN : 2757-7163

Activity of Diosmin-Hesperidin in Kidney Ischemia-reperfusion Injury in Rats: Experimental Study
Kenan Yalcin1, Erim Ersoy2
1Department of Urology, Tokat Medical Park Hospital, Tokat, Türkiye
2Department of Urology, University of Health Sciences, Ankara Training and Research Hospital, Ankara, Türkiye
DOI : 10.5505/GJU.2024.07078
Pages : 012-018
Objective: We aimed to investigate the activity of diosmin-hesperidin (DAFLON®) in ischemia-reperfusion injury in the rat kidney.

Materials and Methods: Twenty-four Wistar rats were used for the experimental study. Each group comprised of 8 subjects. Sham group (Group 1) was nephrectomized for histopathologic examination and blood samples were obtained for biochemical analysis. Control group (Group 2) was subjected to ischemia for 1 hour and reperfusion for 24 hours, then they were nephrectomized for histopathologic examination and blood samples were taken for biochemical analysis. Treatment group (Group 3) was given 80 mg/kg diosminhesperidin combination (DAFLON®) for 10 days and subjected to ischemia for 1-hour and reperfusion for 24 hours, then they were nephrectomized for histopathologic examination and blood samples were obtained for biochemical analysis.

Results: As a result of biochemical analysis and histopathologic examination, more significant results were acquired in regard to other groups. Serum urea values were statistically significant (p<0.05). Consistent with the results of the studies previously performed, higher creatinine values were detected in the control and treatment groups. Enzymatic activities of superoxide dismutase and glutathione peroxidase were significantly lower in the treatment group when compared with the control group. When groups were compared based on histopathologic examination findings, cell necrosis and ischemic alterations were statistically significant parameters (p<0.05). When all parameters were analysed, values indicating any histopathologic abnormalities were at the lowest level in the treatment group. Histopathologic alterations were most frequently detected in the control group.

Conclusion: Anti-inflammatory activity of diosmin-hesperidin which is used experimentally in the treatment of ischemia-reperfusion injury was evaluated both biochemically and histopathologically. Based on the literature data, it is thought that the damage occurring after ischemia-reperfusion injury can be prevented with diosmin-hesperidin treatment.

Keywords : kidney, ischemia-reperfusion injury, diosmin-hesperidin
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